Health Workers (HWs) are most likely to be infected by the Ebola virus disease (EVD) through mainly nosocomial transmission because they treat patients with EVD; this occurs through close contact with patients when infection prevention and control (IPC) measures are not strictly practiced. This study aimed to assess the level of implementation of infection prevention and control measures as well as challenges in their implementation in the north-eastern region of the Democratic Republic of the Congo (DRC). A mixed study was carried out in northeastern of DRC in August 2019. In-depth interviews were conducted with 16 HCW in front-line and with a member of the national coordination team. The quantitative part was focused on the review of the reports of the commission for the prevention and control of infections. Interviews were audiorecorded and transcribed verbatim in Swahili before being translated in French and in English. The IPC packages were implemented in the health Facilities and in the community. We noticed a high proportion of realization of the IPC planned activities. All HCWs interviewed declared to have been trained on IPC and supported with materials and equipment for implementation. Cultural and traditional views of community have been mentioned as the community related factor which impact negatively in IPC implementation by the HCWs. Non-compliance with IPC measures by HCWs has been also mentioned added with the non viability of healthcare facilities. It is urgent to improve communication of importance to comply with IPC measures in community but also among HCWs. Also, Infrastructure of Health Care Facility must be strengthened.